diff --git a/dev-python/tridesclous/ChangeLog b/dev-python/tridesclous/ChangeLog
new file mode 100644
index 0000000..f4dbc91
--- /dev/null
+++ b/dev-python/tridesclous/ChangeLog
@@ -0,0 +1,4 @@
+*tridesclous-1.6.4 (12 Oct 2021)
+
+  12 Oct 2021;  <chymera@gentoo.org> +metadata.xml, +tridesclous-1.6.4.ebuild:
+  dev-python/tridesclous: new package (DANDI ephys stack)
diff --git a/dev-python/tridesclous/Manifest b/dev-python/tridesclous/Manifest
new file mode 100644
index 0000000..da06e2e
--- /dev/null
+++ b/dev-python/tridesclous/Manifest
@@ -0,0 +1 @@
+DIST tridesclous-1.6.4.tar.gz 11798060 SHA256 4ef93660b4752744bcaf3db5d0925a417446295098657370080608bda08fe0dd SHA512 afb50bbbe51fa57fbe7479576c8bebefbd56d8bc135ec38362cdf8b9e51cb4da990d7262e653a9636b1a7b8e45d4f9c4700a37884a235958978b75b968be2a19 WHIRLPOOL b862fc267cc8bda22a4b870f7fd427bac3299bbafa324fd82e60d341f1e8f1182a42cececde3a13f0ed8f6f6650b960fa58bf873f2f6b18be634a8b14e8e521c
diff --git a/dev-python/tridesclous/metadata.xml b/dev-python/tridesclous/metadata.xml
new file mode 100644
index 0000000..78cd54e
--- /dev/null
+++ b/dev-python/tridesclous/metadata.xml
@@ -0,0 +1,15 @@
+<?xml version='1.0' encoding='UTF-8'?>
+<!DOCTYPE pkgmetadata SYSTEM "http://www.gentoo.org/dtd/metadata.dtd">
+<pkgmetadata>
+	<maintainer type="person">
+		<email>gentoo@chymera.eu</email>
+		<name>Horea Christian</name>
+	</maintainer>
+	<maintainer type="project">
+		<email>sci@gentoo.org</email>
+		<name>Gentoo Science Project</name>
+	</maintainer>
+	<upstream>
+		<remote-id type="github">tridesclous/tridesclous</remote-id>
+	</upstream>
+</pkgmetadata>
diff --git a/dev-python/tridesclous/tridesclous-1.6.4.ebuild b/dev-python/tridesclous/tridesclous-1.6.4.ebuild
new file mode 100644
index 0000000..0e18ed5
--- /dev/null
+++ b/dev-python/tridesclous/tridesclous-1.6.4.ebuild
@@ -0,0 +1,67 @@
+# Copyright 2021 Gentoo Authors
+# Distributed under the terms of the GNU General Public License v2
+
+EAPI=8
+
+PYTHON_COMPAT=( python3_{8..10} )
+inherit distutils-r1
+
+DESCRIPTION="offline/online spike sorting"
+HOMEPAGE="https://github.com/tridesclous/tridesclous"
+SRC_URI="https://github.com/tridesclous/tridesclous/archive/refs/tags/${PV}.tar.gz -> ${P}.tar.gz"
+
+LICENSE="MIT"
+SLOT="0"
+KEYWORDS="~amd64"
+IUSE="gui test"
+RESTRICT="test"
+# Tests fail, reported upstream
+# https://github.com/tridesclous/tridesclous/issues/175
+
+RDEPEND="
+	dev-python/cython[${PYTHON_USEDEP}]
+	dev-python/hdbscan[${PYTHON_USEDEP}]
+	dev-python/loky[${PYTHON_USEDEP}]
+	dev-python/matplotlib[${PYTHON_USEDEP}]
+	dev-python/neo[${PYTHON_USEDEP}]
+	dev-python/numba
+	dev-python/numpy[${PYTHON_USEDEP}]
+	dev-python/openpyxl[${PYTHON_USEDEP}]
+	dev-python/pandas[${PYTHON_USEDEP}]
+	dev-python/scipy[${PYTHON_USEDEP}]
+	dev-python/tqdm[${PYTHON_USEDEP}]
+	sci-libs/scikit-learn[${PYTHON_USEDEP}]
+	gui? (
+		dev-python/PyQt5[${PYTHON_USEDEP}]
+	)
+"
+# Technically also seaborn:
+# dev-python/seaborn[${PYTHON_USEDEP}]
+DEPEND="
+	test? (
+		${RDEPEND}
+	)
+"
+
+distutils_enable_tests pytest
+
+python_test() {
+	local EPYTEST_IGNORE=(
+		tridesclous/online/tests/test_launcher.py
+		tridesclous/online/tests/test_onlinepeeler.py
+		tridesclous/online/tests/test_onlinewindow.py
+	)
+	if use !gui ; then
+		EPYTEST_IGNORE+=(
+			tridesclous/gui/test_gpuselector.py
+			tridesclous/gui/icons/test_ressources.py
+			tridesclous/gui/tests/test_cataloguewindow.py
+			tridesclous/gui/tests/test_gui_tools.py
+			tridesclous/gui/tests/test_initializedatasetwindow.py
+			tridesclous/gui/tests/test_mainwindow.py
+			tridesclous/gui/tests/test_peelerwindow.py
+			tridesclous/gui/tests/test_probegeometryview.py
+		)
+	fi
+	epytest
+}